A standard curve

Plasma Proteins- Part II MEASURING PLASMA PROTEIN CONCENTRATION: Part II Learning Outcomes Upon completion of this lab, students should be able to 1. Calculate dilutions to be used to create a standard curve. 2. Draw a standard curve on graph paper using Excel. 3. Use a standard curve to identify the concentration of an unknown. Terminology 0 Micropipette 0 Spectrometer 0 Calibrate Introduction In Part II of the plasma protein lab, you will be creating your own standard curve that you will use to determine the albumin concentration of an unknown sample. The y—axis will be labeled with four albumin concentrations, from 0.5 mg/ml to 2.0 mg/dL. You will be provided with a stock solution of 2.0 mg/ml of BSA that will need to be diluted to 1.5, 1.0, and 0.5 mg/dL. KCI will be used to dilute the stock solution to the desired concentration. Dilution Table: Refer to Part | for the formula to complete the dilution table below. Calculate how much stock solution (2 mg/ml BSA) and how much 0.5 M KCI (diluent) you need to mix to obtain the desired dilutions. Write the volumes needed into the appropriate spaces in the dilution table below. Table 1: Dilution Table to Make Various Concentrations of BSA Desired BSA Amount of stock solution (2 mg/ml BSA) Amount of diluent (0.5 M KCI) needed Concentration needed to make 1.5 ml of the dilution to make 1.5 ml of the dilution