Mutation in astrocytes

Bryan’s Disease arises from a genetic mutation in astrocytes.  You want to take skin cells from a patient with Bryan’s Disease, reprogram them into induced pluripotent stem cells (iPSCs), correct the mutation with CRISPR Cas9, differentiate these cells into astrocyte progenitor cells, inject these astrocyte progenitor cells into a mouse brain and have them differentiate into astrocytes. Show that 30% of your cells were infected with virus and 50% of injected cells turned into astrocytes.

Note: you will make it in a mouse without an immune system so that the mouse does not mount an immune response to the injected human cells.  Please draw your experimental design using a flow chart with arrows on a clean sheet of paper.  Once you have drawn it, take a picture of it or turn it into a PDF and upload it here.  If you cannot upload it here, upload it to the assignment titled, “Upload exam pictures here.” GFAP is a marker for astrocytes; APE is a marker for astrocyte progenitor cells.