Project #3 Staphylococcus Unknown Flow Diagram Objective: . Using selected physiological traits and a list of coagulase negative Staphylococcus species, students will produce a flow diagram separating all possible Staphylococcus species.

Overview: In this handout, students will have a list of the species of genus Staphylococcus that are coagulase negative.

The coagulase test is used to separate the genus Staphylococcus into two groups, either coagulase positive or coagulase negative.

It is not necessary at this time to perform and interpret the coagulase test, however we will do so in lab 13. Subsequent pages of this handout include

1) A list of the possible unknown Staphylococcus species, and

2) A list of tests and traits that cannot be performed or used to determine the identity of the Staphylococcus unknown.

3) Following an introduction to Bergey’s Manual of Determinative Bacteriology and an introduction to flow diagrams, students will produce and turn in a flow diagram that separates the possible Staphylococcus unknown species.

This flow diagram will be used to complete a later assignment called Staphylococcus Unknown ID. Students will be expected to use Table 17.5 in Bergey’s Manual of Determinative Bacteriology to produce their flow diagram.

This book is located in the lab, and two copies are available at the Reserve Desk in the library at the Rancho Campus. You will be using a copy of this table (see handout).

1. List of Possible Staphylococcus Unknowns The list of Staphylococcus species shown below are all coagulase negative Staphylococcus species that can cause infections in humans.

In contrast, Staphylococcus aureus is coagulase positive and is not included in the list. S. capitis subsp. capitis S. hominis S. schleiferi subsp. schleiferi S. capitis subsp.

ureolyticus S. lentus S. sciuri S. cohnii subsp. cohnii S. lugdumensis S. simulans

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