The relationship between missense mutation and cancer

in a tumor cell line, we isolate gene x which has a single missense mutation compared to the WT gene. You isolate the WT of Genex and call it x-gene x. when we delete gene x from the tumor cells this makes cell division slow, and cells stop growing independent of anchorage. When we delete both copies of x-gene x this causes cells to die. How can we test the relationship between missense mutation and cancer?

1. fuse x- gene x to gfp express it in normal cells and monitor its localization by fluences micro.

2. fuse x-gene x and gene x to GFP, express them in normal cells, and compare their localization patterns

3. show that the missense mutation is sufficient by introducing the identical mutation into x-gene x expressing it in a noncancer cell line and monitoring cancer phenotypes

4. express the protein with the missense mutation purify it and show that it has biochemical activity in a test tube

5. show that the missense mutation is sufficient by converting the codon back to wild type expressing it in a noncancer line and monitoring cancer phenotypes.