Sample solution containing lipids.

Sample solution containing lipids. Started my lab but don’t understand what would be the proper way for the conclusion as many of my hypotheses were wrong. As well how to properly answer the questions

Part 2: Testing for Lipids

Background Information:

Lipids are a class of molecules that are not soluble (do not dissolve) in water. They are composed of molecular building blocks of glycerol and three fatty acids. Fatty acids come in two major types, saturated and unsaturated. This difference is due to the presence of particular types of bonds within the fatty acid molecule and affects the shape and characteristics of the overall lipid-containing these fatty acids. Today we will be performing the Paper Test to identify the presence of lipids. The paper turns translucent (gets a grease spot) in presence of lipids.

Question: Which sample solution will contain lipids?

Hypothesis: Hypothesize what will be which solution will have lipids present in the sample (no lipids, some lipids, etc), if any, for each solution. Record your prediction in Table 2 below.

Materials:

• Distilled water
• Vegetable oil
• Hand cream diluted in water
• Shampoo diluted in water
• Brown paper bag
• 4 Disposable pipets
• Timer
• Paper and Pencil

Procedure:

1. Draw four circles in a brown paper bag and label them: Water, Oil, Cream, and Shampoo.
2. Put two drops of each corresponding substance on a brown paper bag.
3. Put the bag off to the side and let it sit for 15 minutes.
4. Record the appearance of the bag where you placed the spots in Table 2.

Results: (Watch the Lab 4. Chemical Composition of Cells video)

Table 2: Results for Testing for Lipids

Conclusion:

1. What are the monomers for lipids?

1. Which type of lipids should we try to avoid eating: saturated or unsaturated? Explain.

1. Which samples were your positive and negative controls?

1. What happens when you mix oil and water? How does this relate to the phospholipid bilayer?