Sickle Cell Anemia Detection Simulation

Sickle Cell Anemia Detection Simulation: PCR, Restriction
Enzyme Digest, and Electrophoresis

Write down the known partial DNA sequence for the wild-type beta-globin gene as indicated in your lab
instructions. Using the information in Table 1, find the recognition site for Dde1 in your written DNA
sequences. Draw a circle around the Dde1 recognition site(s) in your DNA sequence, and draw a bold line
where Dde1 would cleave. Remember, Dde1 will only cut where there is an exact match in sequence.

Write down the known partial DNA sequence for the DNA sequence for the known mutation associated
with sickle cell anemia as indicated in your lab instructions. Using the information in Table 1, find the
recognition site for Dde1 in your written DNA sequences. Draw a circle around the Dde1 recognition site(s) in
your DNA sequence, and draw a bold line where Dde1 would cleave. Remember, Dde1 will only cut where
there is an exact match in sequence.

Draw bands on the gel diagram in Figure 4 representing DNA samples from individuals with different sets of
beta-globin alleles. Draw the bands for each sample: wild-type, heterozygous mutant, and homozygous
mutant (see the lab instructions for step by step procedure).